Claudia Litterst
Established PhD research leader with a proven track record of developing innovative assays for the biotech industry
Research Interests
About
Publications
Functional Characterization of Multiple Transactivating Elements in β-Catenin, Some of Which Interact with the TATA-binding Proteinin Vitro
Journal of Biological Chemistry / Jun 01, 1999
Hecht, A., Litterst, C. M., Huber, O., & Kemler, R. (1999). Functional Characterization of Multiple Transactivating Elements in β-Catenin, Some of Which Interact with the TATA-binding Proteinin Vitro. Journal of Biological Chemistry, 274(25), 18017–18025. https://doi.org/10.1074/jbc.274.25.18017
Metalloproteinase/Presenilin1 processing of ephrinB regulates EphB-induced Src phosphorylation and signaling
The EMBO Journal / Mar 02, 2006
Georgakopoulos, A., Litterst, C., Ghersi, E., Baki, L., Xu, C., Serban, G., & Robakis, N. K. (2006). Metalloproteinase/Presenilin1 processing of ephrinB regulates EphB-induced Src phosphorylation and signaling. The EMBO Journal, 25(6), 1242–1252. https://doi.org/10.1038/sj.emboj.7601031
Multiplexed Target Detection Using DNA-Binding Dye Chemistry in Droplet Digital PCR
Analytical Chemistry / Nov 19, 2013
McDermott, G. P., Do, D., Litterst, C. M., Maar, D., Hindson, C. M., Steenblock, E. R., Legler, T. C., Jouvenot, Y., Marrs, S. H., Bemis, A., Shah, P., Wong, J., Wang, S., Sally, D., Javier, L., Dinio, T., Han, C., Brackbill, T. P., Hodges, S. P., … Lowe, A. J. (2013). Multiplexed Target Detection Using DNA-Binding Dye Chemistry in Droplet Digital PCR. Analytical Chemistry, 85(23), 11619–11627. https://doi.org/10.1021/ac403061n
Akt-Mediated Phosphorylation of Argonaute 2 Downregulates Cleavage and Upregulates Translational Repression of MicroRNA Targets
Molecular Cell / May 01, 2013
Horman, S. R., Janas, M. M., Litterst, C., Wang, B., MacRae, I. J., Sever, M. J., Morrissey, D. V., Graves, P., Luo, B., Umesalma, S., Qi, H. H., Miraglia, L. J., Novina, C. D., & Orth, A. P. (2013). Akt-Mediated Phosphorylation of Argonaute 2 Downregulates Cleavage and Upregulates Translational Repression of MicroRNA Targets. Molecular Cell, 50(3), 356–367. https://doi.org/10.1016/j.molcel.2013.03.015
Ligand Binding and Calcium Influx Induce Distinct Ectodomain/γ-Secretase-processing Pathways of EphB2 Receptor
Journal of Biological Chemistry / Jun 01, 2007
Litterst, C., Georgakopoulos, A., Shioi, J., Ghersi, E., Wisniewski, T., Wang, R., Ludwig, A., & Robakis, N. K. (2007). Ligand Binding and Calcium Influx Induce Distinct Ectodomain/γ-Secretase-processing Pathways of EphB2 Receptor. Journal of Biological Chemistry, 282(22), 16155–16163. https://doi.org/10.1074/jbc.m611449200
Quantitative telomerase enzyme activity determination using droplet digital PCR with single cell resolution
Nucleic Acids Research / May 26, 2014
Ludlow, A. T., Robin, J. D., Sayed, M., Litterst, C. M., Shelton, D. N., Shay, J. W., & Wright, W. E. (2014). Quantitative telomerase enzyme activity determination using droplet digital PCR with single cell resolution. Nucleic Acids Research, 42(13), e104–e104. https://doi.org/10.1093/nar/gku439
FAD mutants unable to increase neurotoxic Aβ 42 suggest that mutation effects on neurodegeneration may be independent of effects on Aβ
Journal of Neurochemistry / Nov 29, 2006
Shioi, J., Georgakopoulos, A., Mehta, P., Kouchi, Z., Litterst, C. M., Baki, L., & Robakis, N. K. (2006). FAD mutants unable to increase neurotoxic Aβ 42 suggest that mutation effects on neurodegeneration may be independent of effects on Aβ. Journal of Neurochemistry, 101(3), 674–681. Portico. https://doi.org/10.1111/j.1471-4159.2006.04391.x
NCoA-1/SRC-1 Is an Essential Coactivator of STAT5 That Binds to the FDL Motif in the α-Helical Region of the STAT5 Transactivation Domain
Journal of Biological Chemistry / Nov 01, 2003
Litterst, C. M., Kliem, S., Marilley, D., & Pfitzner, E. (2003). NCoA-1/SRC-1 Is an Essential Coactivator of STAT5 That Binds to the FDL Motif in the α-Helical Region of the STAT5 Transactivation Domain. Journal of Biological Chemistry, 278(46), 45340–45351. https://doi.org/10.1074/jbc.m303644200
Structure of the NCoA-1/SRC-1 PAS-B Domain Bound to the LXXLL Motif of the STAT6 Transactivation Domain
Journal of Molecular Biology / Feb 01, 2004
Razeto, A., Ramakrishnan, V., Litterst, C. M., Giller, K., Griesinger, C., Carlomagno, T., Lakomek, N., Heimburg, T., Lodrini, M., Pfitzner, E., & Becker, S. (2004). Structure of the NCoA-1/SRC-1 PAS-B Domain Bound to the LXXLL Motif of the STAT6 Transactivation Domain. Journal of Molecular Biology, 336(2), 319–329. https://doi.org/10.1016/j.jmb.2003.12.057
Transcriptional Activation by STAT6 Requires the Direct Interaction with NCoA-1
Journal of Biological Chemistry / Dec 01, 2001
Litterst, C. M., & Pfitzner, E. (2001). Transcriptional Activation by STAT6 Requires the Direct Interaction with NCoA-1. Journal of Biological Chemistry, 276(49), 45713–45721. https://doi.org/10.1074/jbc.m108132200
An LXXLL Motif in the Transactivation Domain of STAT6 Mediates Recruitment of NCoA-1/SRC-1
Journal of Biological Chemistry / Sep 01, 2002
Litterst, C. M., & Pfitzner, E. (2002). An LXXLL Motif in the Transactivation Domain of STAT6 Mediates Recruitment of NCoA-1/SRC-1. Journal of Biological Chemistry, 277(39), 36052–36060. https://doi.org/10.1074/jbc.m203556200
Cadherins Mediate Both the Association between PS1 and β-Catenin and the Effects of PS1 on β-Catenin Stability
Journal of Biological Chemistry / Oct 01, 2005
Serban, G., Kouchi, Z., Baki, L., Georgakopoulos, A., Litterst, C. M., Shioi, J., & Robakis, N. K. (2005). Cadherins Mediate Both the Association between PS1 and β-Catenin and the Effects of PS1 on β-Catenin Stability. Journal of Biological Chemistry, 280(43), 36007–36012. https://doi.org/10.1074/jbc.m507503200
Peptide EphB2/CTF2 Generated by the γ-Secretase Processing of EphB2 Receptor Promotes Tyrosine Phosphorylation and Cell Surface Localization of N-Methyl-d-aspartate Receptors
Journal of Biological Chemistry / Oct 01, 2009
Xu, J., Litterst, C., Georgakopoulos, A., Zaganas, I., & Robakis, N. K. (2009). Peptide EphB2/CTF2 Generated by the γ-Secretase Processing of EphB2 Receptor Promotes Tyrosine Phosphorylation and Cell Surface Localization of N-Methyl-d-aspartate Receptors. Journal of Biological Chemistry, 284(40), 27220–27228. https://doi.org/10.1074/jbc.m109.048728
Coactivators in Gene Regulation by STAT5
Vitamins & Hormones / Jan 01, 2005
Litterst, C. M., Kliem, S., Lodrini, M., & Pfitzner, E. (2005). Coactivators in Gene Regulation by STAT5. In Vitamins & Hormones (pp. 359–386). Elsevier. https://doi.org/10.1016/s0083-6729(05)70012-1
MZC Gel Inhibits SHIV-RT and HSV-2 in Macaque Vaginal Mucosa and SHIV-RT in Rectal Mucosa
JAIDS Journal of Acquired Immune Deficiency Syndromes / Mar 01, 2017
Calenda, G., Villegas, G., Barnable, P., Litterst, C., Levendosky, K., Gettie, A., Cooney, M. L., Blanchard, J., Fernández-Romero, J. A., Zydowsky, T. M., & Teleshova, N. (2017). MZC Gel Inhibits SHIV-RT and HSV-2 in Macaque Vaginal Mucosa and SHIV-RT in Rectal Mucosa. JAIDS Journal of Acquired Immune Deficiency Syndromes, 74(3), e67–e74. https://doi.org/10.1097/qai.0000000000001167
Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCRTM Assays
Methods in Molecular Biology / Jan 01, 2018
Karlin-Neumann, G., Zhang, B., & Litterst, C. (2018). Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCRTM Assays. In Digital PCR (pp. 423–444). Springer New York. https://doi.org/10.1007/978-1-4939-7778-9_24
Interaction of STAT6 with its co-activator SRC-1/NCoA-1 is regulated by dephosphorylation of the latter via PP2A
Nucleic Acids Research / Dec 08, 2010
Münz, T., Litterst, C. M., & Pfitzner, E. (2010). Interaction of STAT6 with its co-activator SRC-1/NCoA-1 is regulated by dephosphorylation of the latter via PP2A. Nucleic Acids Research, 39(8), 3255–3266. https://doi.org/10.1093/nar/gkq1225
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Journal of Visualized Experiments / Apr 14, 2010
McCoy, A. M., Litterst, C., Collins, M. L., & Ugozzoli, L. A. (2010). Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells. Journal of Visualized Experiments, 38. https://doi.org/10.3791/1904-v
Abstract 2301: Cross validation of NGS methylated targets using droplet digital PCR (ddPCR)
Cancer Research / Oct 01, 2014
Shelton, D. N., Litterst, C., Regan, J. F., Moinova, H. R., & Markowitz, S. D. (2014). Abstract 2301: Cross validation of NGS methylated targets using droplet digital PCR (ddPCR). Cancer Research, 74(19_Supplement), 2301–2301. https://doi.org/10.1158/1538-7445.am2014-2301
Abstract 5156: Multiplexed EGFR mutation detection with C797S and T790M allelic context using six-color digital PCR
Cancer Research / Jun 15, 2022
Yang, W., Christensen, M., Chan, J. K., Tsai, J., Patten, N., Tran, H. B., Hillman, G. R., Tai, Y. C., Bogard, P., Litterst, C. M., Brophy, V. H., Newton, N., Nelson, C. D., & Pattje, W. J. (2022). Abstract 5156: Multiplexed EGFR mutation detection with C797S and T790M allelic context using six-color digital PCR. Cancer Research, 82(12_Supplement), 5156–5156. https://doi.org/10.1158/1538-7445.am2022-5156
Abstract 5156: Multiplexed EGFR mutation detection with C797S and T790M allelic context using six-color digital PCR
Cancer Research / Jun 15, 2022
Yang, W., Christensen, M., Chan, J. K., Tsai, J., Patten, N., Tran, H. B., Hillman, G. R., Tai, Y. C., Bogard, P., Litterst, C. M., Brophy, V. H., Newton, N., Nelson, C. D., & Pattje, W. J. (2022). Abstract 5156: Multiplexed EGFR mutation detection with C797S and T790M allelic context using six-color digital PCR. Cancer Research, 82(12_Supplement), 5156–5156. https://doi.org/10.1158/1538-7445.am2022-5156
Abstract P6-07-20: Allele-specific PCR assay for estrogen receptor (ESR1) mutation detection
Cancer Research / Feb 15, 2017
Litterst, C., Tran, H., Chien, S., Chen, X., Wen, W., & Begovich, A. (2017). Abstract P6-07-20: Allele-specific PCR assay for estrogen receptor (ESR1) mutation detection. Cancer Research, 77(4_Supplement), P6-07-20-P6-07–20. https://doi.org/10.1158/1538-7445.sabcs16-p6-07-20
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Journal of Visualized Experiments / Apr 14, 2010
McCoy, A. M., Litterst, C., Collins, M. L., & Ugozzoli, L. A. (2010). Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells. Journal of Visualized Experiments, 38. https://doi.org/10.3791/1904
O3–03–02: PS1/γ–secretase regulate the EphB–induced Src signaling
Alzheimer's & Dementia / Jul 01, 2006
Georgakopoulos, A., Litterst, C., Ghersi, E., Baki, L., Xu, C., Serban, G., & Robakis, N. K. (2006). O3–03–02: PS1/γ–secretase regulate the EphB–induced Src signaling. Alzheimer’s & Dementia, 2(3S_Part_2). Portico. https://doi.org/10.1016/j.jalz.2006.05.206
P4-301 Presenilin-1/gamma secretase cleavage of ephrinb ligands and EPHB receptors
Neurobiology of Aging / Jul 01, 2004
Georgakopoulos, A., Litterst, C., Xu, C.-J., Wen, P., Shao, Z., & Robakis, N. (2004). P4-301 Presenilin-1/gamma secretase cleavage of ephrinb ligands and EPHB receptors. Neurobiology of Aging, 25, S560–S561. https://doi.org/10.1016/s0197-4580(04)81859-1
P3-330 γ-secretase processing of VE-cadherin regulates CBP-mediated transcription
Neurobiology of Aging / Jul 01, 2004
Serban, G., Marambaud, P., Litterst, C., & Robakis, N. (2004). P3-330 γ-secretase processing of VE-cadherin regulates CBP-mediated transcription. Neurobiology of Aging, 25, S449. https://doi.org/10.1016/s0197-4580(04)81480-5
Education
Goethe University Frankfurt
Ph.D., Biology / November, 2002
Albert Ludwig University of Freiburg
Master of Science, Biology / March, 1999
Experience
Roche
Research Leader and Principal Scientist II / June, 2015 — October, 2023
- Led the development of PCR-based assays for cancer, chronic liver and infectious disease biomarker and diagnostics. - Oversaw the early development of digital PCR reagents. - Led a team of non-PhD and PhD level scientists.
Bio-Rad Laboratories
Staff Scientist and Senior Scientist / 2008 — 2015
- Led the validation of new PCR reagents for the QX200 droplet digital PCR system. - Developed new applications for Bio-Rad reagents and instrumentation including mammalian cell transfection and PCR. - Published protocols for new applications in peer reviewed articles, technical reports and successfully filed patent applications.
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