Murli Manohar
Boyce Thompson Institute, Cornell University
Research Expertise
About
Publications
Conserved nematode signalling molecules elicit plant defenses and pathogen resistance
Nature Communications / Jul 23, 2015
Manosalva, P., Manohar, M., von Reuss, S. H., Chen, S., Koch, A., Kaplan, F., Choe, A., Micikas, R. J., Wang, X., Kogel, K.-H., Sternberg, P. W., Williamson, V. M., Schroeder, F. C., & Klessig, D. F. (2015). Conserved nematode signalling molecules elicit plant defenses and pathogen resistance. Nature Communications, 6(1). https://doi.org/10.1038/ncomms8795
Identification of multiple salicylic acid-binding proteins using two high throughput screens
Frontiers in Plant Science / Jan 12, 2015
Manohar, M., Tian, M., Moreau, M., Park, S.-W., Choi, H. W., Fei, Z., Friso, G., Asif, M., Manosalva, P., von Dahl, C. C., Shi, K., Ma, S., Dinesh-Kumar, S. P., O’Doherty, I., Schroeder, F. C., van Wijk, K. J., & Klessig, D. F. (2015). Identification of multiple salicylic acid-binding proteins using two high throughput screens. Frontiers in Plant Science, 5. https://doi.org/10.3389/fpls.2014.00777
Plant cation/H+ exchangers (CAXs): biological functions and genetic manipulations
Plant Biology / May 12, 2011
Manohar, M., Shigaki, T., & Hirschi, K. D. (2011). Plant cation/H+ exchangers (CAXs): biological functions and genetic manipulations. Plant Biology, 13(4), 561–569. Portico. https://doi.org/10.1111/j.1438-8677.2011.00466.x
Chickpea breeding and management
Jan 01, 2007
Yadav, S. S., Redden, R. J., Chen, W., & Sharma, B. (Eds.). (2007). Chickpea breeding and management. CABI. https://doi.org/10.1079/9781845932138.000
Lentil
Jan 01, 2007
Yadav, S. S., McNeil, D. L., & Stevenson, P. C. (Eds.). (2007). Lentil: An Ancient Crop for Modern Times. Springer Netherlands. https://doi.org/10.1007/978-1-4020-6313-8
Aspirin’s Active Metabolite Salicylic Acid Targets High Mobility Group Box 1 to Modulate Inflammatory Responses
Molecular Medicine / Jan 01, 2015
Choi, H. W., Tian, M., Song, F., Venereau, E., Preti, A., Park, S.-W., Hamilton, K., Swapna, G. V. T., Manohar, M., Moreau, M., Agresti, A., Gorzanelli, A., De Marchis, F., Wang, H., Antonyak, M., Micikas, R. J., Gentile, D. R., Cerione, R. A., Schroeder, F. C., … Klessig, D. F. (2015). Aspirin’s Active Metabolite Salicylic Acid Targets High Mobility Group Box 1 to Modulate Inflammatory Responses. Molecular Medicine, 21(1), 526–535. https://doi.org/10.2119/molmed.2015.00148
Activation of Plant Innate Immunity by Extracellular High Mobility Group Box 3 and Its Inhibition by Salicylic Acid
PLOS Pathogens / Mar 23, 2016
Choi, H. W., Manohar, M., Manosalva, P., Tian, M., Moreau, M., & Klessig, D. F. (2016). Activation of Plant Innate Immunity by Extracellular High Mobility Group Box 3 and Its Inhibition by Salicylic Acid. PLOS Pathogens, 12(3), e1005518. https://doi.org/10.1371/journal.ppat.1005518
Plant metabolism of nematode pheromones mediates plant-nematode interactions
Nature Communications / Jan 10, 2020
Manohar, M., Tenjo-Castano, F., Chen, S., Zhang, Y. K., Kumari, A., Williamson, V. M., Wang, X., Klessig, D. F., & Schroeder, F. C. (2020). Plant metabolism of nematode pheromones mediates plant-nematode interactions. Nature Communications, 11(1). https://doi.org/10.1038/s41467-019-14104-2
Human GAPDH Is a Target of Aspirin’s Primary Metabolite Salicylic Acid and Its Derivatives
PLOS ONE / Nov 25, 2015
Choi, H. W., Tian, M., Manohar, M., Harraz, M. M., Park, S.-W., Schroeder, F. C., Snyder, S. H., & Klessig, D. F. (2015). Human GAPDH Is a Target of Aspirin’s Primary Metabolite Salicylic Acid and Its Derivatives. PLOS ONE, 10(11), e0143447. https://doi.org/10.1371/journal.pone.0143447
Calcium transport from source to sink: understanding the mechanism(s) of acquisition, translocation, and accumulation for crop biofortification
Acta Physiologiae Plantarum / Dec 07, 2014
Kumar, A., Singh, U. M., Manohar, M., & Gaur, V. S. (2014). Calcium transport from source to sink: understanding the mechanism(s) of acquisition, translocation, and accumulation for crop biofortification. Acta Physiologiae Plantarum, 37(1). https://doi.org/10.1007/s11738-014-1722-6
Uses, consumption and utilization.
Chickpea breeding and management / Jan 01, 2007
Yadav, S. S., Longnecker, N., Dusunceli, F., Bejiga, G., Yadav, M., Rizvi, A. H., Manohar, M., Reddy, A. A., Xaxiao, Z., & Chen, W. (2007). Uses, consumption and utilization. In Chickpea breeding and management (pp. 72–100). CABI. https://doi.org/10.1079/9781845932138.004
Members of the abscisic acid co‐receptor
PP 2C protein family mediate
salicylic acid–abscisic acid crosstalk
Plant Direct / Nov 01, 2017
Manohar, M., Wang, D., Manosalva, P. M., Choi, H. W., Kombrink, E., & Klessig, D. F. (2017). Members of the abscisic acid co‐receptor <scp>PP</scp>2C protein family mediate salicylic acid–abscisic acid crosstalk. Plant Direct, 1(5). Portico. https://doi.org/10.1002/pld3.20
Uses and Consumption
Lentil / Jan 01, 2007
Yadav, S. S., Stevenson, P. C., Rizvi, A. H., Manohar, M., Gailing, S., & Mateljan, G. (2007). Uses and Consumption. In Lentil (pp. 33–46). Springer Netherlands. https://doi.org/10.1007/978-1-4020-6313-8_4
Plant and Human MORC Proteins Have DNA-Modifying Activities Similar to Type II Topoisomerases, but Require One or More Additional Factors for Full Activity
Molecular Plant-Microbe Interactions® / Feb 01, 2017
Manohar, M., Choi, H. W., Manosalva, P., Austin, C. A., Peters, J. E., & Klessig, D. F. (2017). Plant and Human MORC Proteins Have DNA-Modifying Activities Similar to Type II Topoisomerases, but Require One or More Additional Factors for Full Activity. Molecular Plant-Microbe Interactions®, 30(2), 87–100. https://doi.org/10.1094/mpmi-10-16-0208-r
Lentil Growers and Production Systems around the World
Lentil / Jan 01, 2007
Yadav, S. S., Rizvi, A. H., Manohar, M., Verma, A. K., Shrestha, R., Chen, Chengci., Bejiga, G., Chen, W., Yadav, M., & Bahl, P. N. (2007). Lentil Growers and Production Systems around the World. In Lentil (pp. 415–442). Springer Netherlands. https://doi.org/10.1007/978-1-4020-6313-8_23
Chickpea Biofortification for Cytokinin Dehydrogenase via Genome Editing to Enhance Abiotic-Biotic Stress Tolerance and Food Security
Frontiers in Genetics / May 20, 2022
Mahto, R. K., Ambika, Singh, C., Chandana, B. S., Singh, R. K., Verma, S., Gahlaut, V., Manohar, M., Yadav, N., & Kumar, R. (2022). Chickpea Biofortification for Cytokinin Dehydrogenase via Genome Editing to Enhance Abiotic-Biotic Stress Tolerance and Food Security. Frontiers in Genetics, 13. https://doi.org/10.3389/fgene.2022.900324
The GHKL ATPase MORC1 Modulates Species-Specific Plant Immunity in Solanaceae
Molecular Plant-Microbe Interactions® / Aug 01, 2015
Manosalva, P., Manohar, M., Kogel, K.-H., Kang, H.-G., & Klessig, D. F. (2015). The GHKL ATPase MORC1 Modulates Species-Specific Plant Immunity in Solanaceae. Molecular Plant-Microbe Interactions®, 28(8), 927–942. https://doi.org/10.1094/mpmi-12-14-0401-r
Nematode Signaling Molecules Are Extensively Metabolized by Animals, Plants, and Microorganisms
ACS Chemical Biology / May 21, 2021
Yu, Y., Zhang, Y. K., Manohar, M., Artyukhin, A. B., Kumari, A., Tenjo-Castano, F. J., Nguyen, H., Routray, P., Choe, A., Klessig, D. F., & Schroeder, F. C. (2021). Nematode Signaling Molecules Are Extensively Metabolized by Animals, Plants, and Microorganisms. ACS Chemical Biology, 16(6), 1050–1058. https://doi.org/10.1021/acschembio.1c00217
Epigenomics as Potential Tools for Enhancing Magnitude of Breeding Approaches for Developing Climate Resilient Chickpea
Frontiers in Genetics / Jul 22, 2022
Chandana, B. S., Mahto, R. K., Singh, R. K., Ford, R., Vaghefi, N., Gupta, S. K., Yadav, H. K., Manohar, M., & Kumar, R. (2022). Epigenomics as Potential Tools for Enhancing Magnitude of Breeding Approaches for Developing Climate Resilient Chickpea. Frontiers in Genetics, 13. https://doi.org/10.3389/fgene.2022.900253
Expression of mouse small interfering RNAs in lettuce using artificial microRNA technology
BioTechniques / Apr 01, 2020
Kakeshpour, T., Tamang, T. M., Park, W. D., Manohar, M., Yang, J., Hirschi, K. D., & Park, S. (2020). Expression of mouse small interfering RNAs in lettuce using artificial microRNA technology. BioTechniques, 68(4), 214–218. https://doi.org/10.2144/btn-2019-0139
Efficacy of Ascaroside #18 Treatments in Control of Salmonella enterica on Alfalfa and Fenugreek Seeds and Sprouts
Journal of Food Protection / Mar 01, 2023
Hu, X., Lee, S., Manohar, M., & Chen, J. (2023). Efficacy of Ascaroside #18 Treatments in Control of Salmonella enterica on Alfalfa and Fenugreek Seeds and Sprouts. Journal of Food Protection, 86(3), 100064. https://doi.org/10.1016/j.jfp.2023.100064
Synthesis and characterization of microporous polymer microspheres with strong cation-exchange character
Reactive and Functional Polymers / Dec 01, 2012
Cormack, P. A. G., Davies, A., & Fontanals, N. (2012). Synthesis and characterization of microporous polymer microspheres with strong cation-exchange character. Reactive and Functional Polymers, 72(12), 939–946. https://doi.org/10.1016/j.reactfunctpolym.2012.08.003
The fate of enterohemorrhagic Escherichia coli on alfalfa and fenugreek seeds and sprouts as affected by ascaroside #18 treatments
Food Bioscience / Apr 01, 2024
Hu, X., Lee, S., Manohar, M., & Chen, J. (2024). The fate of enterohemorrhagic Escherichia coli on alfalfa and fenugreek seeds and sprouts as affected by ascaroside #18 treatments. Food Bioscience, 58, 103633. https://doi.org/10.1016/j.fbio.2024.103633
Cover Image
Journal of Phytopathology / Apr 16, 2019
Klessig, D. F., Manohar, M., Baby, S., Koch, A., Danquah, W. B., Luna, E., Park, H., Kolkman, J. M., Turgeon, B. G., Nelson, R., Leach, J. E., Williamson, V. M., Kogel, K., Kachroo, A., & Schroeder, F. C. (2019). Cover Image. Journal of Phytopathology, 167(5). Portico. https://doi.org/10.1111/jph.12811
Nematode ascaroside enhances resistance in a broad spectrum of plant–pathogen systems
Journal of Phytopathology / Mar 18, 2019
Klessig, D. F., Manohar, M., Baby, S., Koch, A., Danquah, W. B., Luna, E., Park, H., Kolkman, J. M., Turgeon, B. G., Nelson, R., Leach, J. E., Williamson, V. M., Kogel, K., Kachroo, A., & Schroeder, F. C. (2019). Nematode ascaroside enhances resistance in a broad spectrum of plant–pathogen systems. Journal of Phytopathology, 167(5), 265–272. Portico. https://doi.org/10.1111/jph.12795
Heterodimerization of Arabidopsis calcium/proton exchangers contributes to regulation of guard cell dynamics and plant defense responses
Journal of Experimental Botany / Jun 22, 2017
Hocking, B., Conn, S. J., Manohar, M., Xu, B., Athman, A., Stancombe, M. A., Webb, A. R., Hirschi, K. D., & Gilliham, M. (2017). Heterodimerization of Arabidopsis calcium/proton exchangers contributes to regulation of guard cell dynamics and plant defense responses. Journal of Experimental Botany, 68(15), 4171–4183. https://doi.org/10.1093/jxb/erx209
The Compromised Recognition of Turnip Crinkle Virus1 Subfamily of Microrchidia ATPases Regulates Disease Resistance in Barley to Biotrophic and Necrotrophic Pathogens
Plant Physiology / Jan 03, 2014
Langen, G., von Einem, S., Koch, A., Imani, J., Pai, S. B., Manohar, M., Ehlers, K., Choi, H. W., Claar, M., Schmidt, R., Mang, H.-G., Bordiya, Y., Kang, H.-G., Klessig, D. F., & Kogel, K.-H. (2014). The Compromised Recognition of Turnip Crinkle Virus1 Subfamily of Microrchidia ATPases Regulates Disease Resistance in Barley to Biotrophic and Necrotrophic Pathogens. Plant Physiology, 164(2), 866–878. https://doi.org/10.1104/pp.113.227488
Characterization of Arabidopsis Ca2+/H+ Exchanger CAX3
Biochemistry / Jun 22, 2011
Manohar, M., Shigaki, T., Mei, H., Park, S., Marshall, J., Aguilar, J., & Hirschi, K. D. (2011). Characterization of Arabidopsis Ca2+/H+ Exchanger CAX3. Biochemistry, 50(28), 6189–6195. https://doi.org/10.1021/bi2003839
The expression of the open reading frame of Arabidopsis CAX1, but not its cDNA, confers metal tolerance in yeast
Plant Biology / Aug 26, 2010
Shigaki, T., Mei, H., Marshall, J., Li, X., Manohar, M., & Hirschi, K. D. (2010). The expression of the open reading frame of Arabidopsis CAX1, but not its cDNA, confers metal tolerance in yeast: Full-length Arabidopsis CAX1 activity in yeast. Plant Biology, 12(6), 935–939. https://doi.org/10.1111/j.1438-8677.2010.00368.x
Zebrafish (Danio rerio) Endomembrane Antiporter Similar to a Yeast Cation/H+ Transporter Is Required for Neural Crest Development
Biochemistry / Jul 14, 2010
Manohar, M., Mei, H., Franklin, A. J., Sweet, E. M., Shigaki, T., Riley, B. B., MacDiarmid, C. W., & Hirschi, K. (2010). Zebrafish (Danio rerio) Endomembrane Antiporter Similar to a Yeast Cation/H+ Transporter Is Required for Neural Crest Development. Biochemistry, 49(31), 6557–6566. https://doi.org/10.1021/bi100362k
Education
Texas A&M University
Biochemistry, Plant Biology, Biotechnology, Molecular Biology / May, 2012
Indian Institute of Technology Roorkee
M.S, Biotechnology / May, 2005
B.S, Agriculture / May, 2003
Experience
Ascribe Bioscience Inc.
CTO / February, 2019 — Present
Execute the company's technological roadmap in alignment with business goals and vision. Collaborate with cross-functional teams to drive innovation and enhance crop protection products. Analyze market trends and competitive landscape to identify new technological advancements and product differentiation opportunities. ● Built strong partnerships with Ag-tech firms, investors, academic scholars, and growers. ● Controlled research and development budget of more than $10M to drive innovation into tangible outcomes. ● Developed independent research laboratory and managed R&D, multi-national field tests, and commercialization plans. ● Conducted market research to enhance product offerings and align with customer requirements.
Boyce Thompson Institute for Plant Research
Senior Scientist / September, 2012 — November, 2020
Supported multidisciplinary teams in assessing complex data sets, identifying patterns, and drawing meaningful conclusions. Led design and execution of experiments, ensuring adherence to scientific protocols and research methodologies. Facilitated collaborative research projects and leveraged resources for mutual benefit. ● Recognized as the inventor of several technologies, delivering a foundation for establishing Ascribe Bioscience. ● Uncovered unique processes of plant and animal responses to external stresses. ● Earned promotion to Senior Scientist in 2017 based on excellent performance. ● Expert in the fields of Molecular Biology, Plant Biotechnology, Crop Protection, and Gene Editing. ● Authored revolutionary research publications in Agriculture, Molecular Biology, Biochemistry, Biotechnology, and Natural Chemistry.
Baylor College of Medicine
Graduate Research Assistant/Postdoctoral Associate / May, 2008 — September, 2012
The objective was to study and optimize CAX-mediated biofortification and phytoremediation in plants. My research investigated the physiological functions, structure-function relationship, and inter- and intra-molecular interactions of calcium transporters cation/proton exchangers known as CAX in plants. My research used Saccharomyces cerevisiae (yeast) as the model system to study CAX transporters and established membrane fractionation and enrichment techniques to evaluate the real-time movement of metals between membranes. Moreover, I also characterized new calcium transporters from both plants and animals. Other experience: Hands-on experience in executing potency assays (binding assays, In vitro, In vivo). Hands-on experience in RNAi technology (Designing, cloning, and heterologous expression of siRNA in plants targeting animal mRNA).
The University of Texas Health Science Center at Houston School of Public Health
Visiting Research Student / September, 2010 — September, 2012
My work objective was to express and purify CAX (Calcium transporter) proteins through heterologous expression and determine their three-dimensional structure using X-ray crystallography. The work involved cloning 15+ CAX proteins from different plant species in E. coli and yeast expression vectors. It also included optimizing and expressing protein production, screening and solubilizing proteins using detergents, and purifying functional proteins through affinity chromatography. The metal transport activity of purified proteins was evaluated by reconstituting the proteins in proteoliposomes, isothermal titration calorimetry (ITC), and in inverted vesicles prepared from protein-expressing E. coli cells. The purified protein underwent crystallization screening to assess the effectiveness of methods, reagents, and other variables in producing high-quality crystals of CAX proteins for 3D structure analysis.
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