Ajay Badhan

Research Biologist, Lethbridge Research Center, Canada

Lethbridge, Alberta, Canada

Research Interests

Animal nutrition
cell wall biosynthesis and its deconstruction
biofuels
Waste Management and Disposal
Renewable Energy, Sustainability and the Environment
Environmental Engineering
Bioengineering
Biotechnology
Applied Microbiology and Biotechnology
Plant Science
Physiology
Genetics
Food Animals
Animal Science and Zoology
Biochemistry
Management, Monitoring, Policy and Law
Food Science
Microbiology
Drug Discovery
Organic Chemistry
Biochemistry (medical)
Analytical Chemistry
Structural Biology
Biophysics
Computer Science Applications
Molecular Biology
Ecology

About

I am a proficient researcher with valuable research and teaching experience acquired at distinguished institutes like Complex Carbohydrate Research Center, US, University of Alberta, Canada, and Lethbridge Research Center (AAFC), Canada. I have been working for past 15 years on multiple projects focused on the economical, environmental and social sustainability of agricultural production. Improvement in livestock performance, productivity, and health by unlocking the microbiome, development of clean technologies, improving agriculture environmental performance, and Increase agro-ecosystem resilience are prime objectives for my research.

Publications

Production of multiple xylanolytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099

Bioresource Technology / Feb 01, 2007

Badhan, A. K., Chadha, B. S., Kaur, J., Saini, H. S., & Bhat, M. K. (2007). Production of multiple xylanolytic and cellulolytic enzymes by thermophilic fungus Myceliophthora sp. IMI 387099. Bioresource Technology, 98(3), 504–510. https://doi.org/10.1016/j.biortech.2006.02.009

Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922

Electronic Journal of Biotechnology / Apr 15, 2007

Kaur, J., Chadha, B. S., Kumar, B. A., Ghatora, S. K., & Saini, H. S. (2007). Purification and characterization of ß-glucosidase from Melanocarpus sp. MTCC 3922. Electronic Journal of Biotechnology, 10(2), 0–0. https://doi.org/10.2225/vol10-issue2-fulltext-4

Development of Cellulosic Secondary Walls in Flax Fibers Requires β-Galactosidase      

Plant Physiology / May 19, 2011

Roach, M. J., Mokshina, N. Y., Badhan, A., Snegireva, A. V., Hobson, N., Deyholos, M. K., & Gorshkova, T. A. (2011). Development of Cellulosic Secondary Walls in Flax Fibers Requires β-Galactosidase      . Plant Physiology, 156(3), 1351–1363. https://doi.org/10.1104/pp.111.172676

Purification and characterization of two endoglucanases from Melanocarpus sp. MTCC 3922

Bioresource Technology / Jan 01, 2007

KAUR, J., CHADHA, B., KUMAR, B., & SAINI, H. (2007). Purification and characterization of two endoglucanases from Melanocarpus sp. MTCC 3922. Bioresource Technology, 98(1), 74–81. https://doi.org/10.1016/j.biortech.2005.11.019

Mining the rumen for fibrolytic feed enzymes

Animal Frontiers / Apr 01, 2016

Ribeiro, G. O., Gruninger, R. J., Badhan, A., & McAllister, T. A. (2016). Mining the rumen for fibrolytic feed enzymes. Animal Frontiers, 6(2), 20–26. https://doi.org/10.2527/af.2016-0019

Identification and characterization of diverse xylanases from thermophilic and thermotolerant fungi

BioResources / Jul 01, 2006

Ghatora, S. K., Chadha, B. S., Badhan, A. K., Saini, H. S., & Bhat, M. K. (2006). Identification and characterization of diverse xylanases from thermophilic and thermotolerant fungi. BioResources, 1(1), 18–33. https://doi.org/10.15376/biores.1.1.18-33

Functionally diverse multiple xylanases of thermophilic fungus Myceliophthora sp. IMI 387099

Enzyme and Microbial Technology / Oct 01, 2004

Badhan, A. K., Chadha, B. S., Sonia, K. G., Saini, H. S., & Bhat, M. K. (2004). Functionally diverse multiple xylanases of thermophilic fungus Myceliophthora sp. IMI 387099. Enzyme and Microbial Technology, 35(5), 460–466. https://doi.org/10.1016/j.enzmictec.2004.07.002

Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility

Biotechnology for Biofuels / Mar 20, 2014

Badhan, A., Jin, L., Wang, Y., Han, S., Kowalczys, K., Brown, D. C., Ayala, C. J., Latoszek-Green, M., Miki, B., Tsang, A., & McAllister, T. (2014). Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility. Biotechnology for Biofuels, 7(1). https://doi.org/10.1186/1754-6834-7-39

Fibre digestion by rumen microbiota — a review of recent metagenomic and metatranscriptomic studies

Canadian Journal of Animal Science / Dec 01, 2019

Terry, S. A., Badhan, A., Wang, Y., Chaves, A. V., & McAllister, T. A. (2019). Fibre digestion by rumen microbiota — a review of recent metagenomic and metatranscriptomic studies. Canadian Journal of Animal Science, 99(4), 678–692. https://doi.org/10.1139/cjas-2019-0024

Formulation of enzyme blends to maximize the hydrolysis of alkaline peroxide pretreated alfalfa hay and barley straw by rumen enzymes and commercial cellulases

BMC Biotechnology / Apr 26, 2014

Badhan, A., Wang, Y., Gruninger, R., Patton, D., Powlowski, J., Tsang, A., & McAllister, T. (2014). Formulation of enzyme blends to maximize the hydrolysis of alkaline peroxide pretreated alfalfa hay and barley straw by rumen enzymes and commercial cellulases. BMC Biotechnology, 14(1). https://doi.org/10.1186/1472-6750-14-31

Identification of glucose tolerant acid active β-glucosidases from thermophilic and thermotolerant fungi

World Journal of Microbiology and Biotechnology / Aug 23, 2007

Sonia, K. G., Chadha, B. S., Badhan, A. K., Saini, H. S., & Bhat, M. K. (2007). Identification of glucose tolerant acid active β-glucosidases from thermophilic and thermotolerant fungi. World Journal of Microbiology and Biotechnology, 24(5), 599–604. https://doi.org/10.1007/s11274-007-9512-6

Two endoxylanases active and stable at alkaline pH from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099

Journal of Biotechnology / Apr 01, 2004

Chadha, B. S., Ajay, B. K., Mellon, F., & Bhat, M. K. (2004). Two endoxylanases active and stable at alkaline pH from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099. Journal of Biotechnology, 109(3), 227–237. https://doi.org/10.1016/j.jbiotec.2003.12.010

New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw1

Journal of Animal Science / Jul 20, 2018

Ribeiro, G. O., Badhan, A., Huang, J., Beauchemin, K. A., Yang, W., Wang, Y., Tsang, A., & McAllister, T. A. (2018). New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw1. Journal of Animal Science, 96(9), 3928–3942. https://doi.org/10.1093/jas/sky251

Identification of novel enzymes to enhance the ruminal digestion of barley straw

Bioresource Technology / Jul 01, 2018

Badhan, A., Ribeiro, G. O., Jones, D. R., Wang, Y., Abbott, D. W., Di Falco, M., Tsang, A., & McAllister, T. A. (2018). Identification of novel enzymes to enhance the ruminal digestion of barley straw. Bioresource Technology, 260, 76–84. https://doi.org/10.1016/j.biortech.2018.03.086

Role of Transglycosylation Products in the Expression of Multiple Xylanases in Myceliophthora sp. IMI 387099

Current Microbiology / May 14, 2007

Badhan, A. K., Chadha, B. S., Kaur, J., Sonia, K. G., Saini, H. S., & Bhat, M. K. (2007). Role of Transglycosylation Products in the Expression of Multiple Xylanases in Myceliophthora sp. IMI 387099. Current Microbiology, 54(6), 405–409. https://doi.org/10.1007/s00284-006-0204-5

Designer Plants for Biofuels: A Review

Current Metabolomics / Mar 02, 2016

Badhan, A., & McAllister, T. (2016). Designer Plants for Biofuels: A Review. Current Metabolomics, 4(1), 49–55. https://doi.org/10.2174/2213235x03666141226213656

Purification of the alkaliphilic xylanases from Myceliophthora sp. IMI 387099 using cellulose-binding domain as an affinity tag

World Journal of Microbiology and Biotechnology / Sep 19, 2007

Badhan, A. K., Chadha, B. S., & Saini, H. S. (2007). Purification of the alkaliphilic xylanases from Myceliophthora sp. IMI 387099 using cellulose-binding domain as an affinity tag. World Journal of Microbiology and Biotechnology, 24(7), 973–981. https://doi.org/10.1007/s11274-007-9561-x

Mechanistic insights into the digestion of complex dietary fibre by the rumen microbiota using combinatorial high-resolution glycomics and transcriptomic analyses

Computational and Structural Biotechnology Journal / Jan 01, 2022

Badhan, A., Low, K. E., Jones, D. R., Xing, X., Milani, M. R. M., Polo, R. O., Klassen, L., Venketachalam, S., Hahn, M. G., Abbott, D. W., & McAllister, T. A. (2022). Mechanistic insights into the digestion of complex dietary fibre by the rumen microbiota using combinatorial high-resolution glycomics and transcriptomic analyses. Computational and Structural Biotechnology Journal, 20, 148–164. https://doi.org/10.1016/j.csbj.2021.12.009

Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting

BioMed Research International / Jan 01, 2015

Badhan, A., Wang, Y.-X., Gruninger, R., Patton, D., Powlowski, J., Tsang, A., & McAllister, T. A. (2015). Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting. BioMed Research International, 2015, 1–13. https://doi.org/10.1155/2015/562952

Proteomic Profiling of the Aleurone Layer of Mature Arabidopsis thaliana Seed

Plant Molecular Biology Reporter / Aug 07, 2012

Rashid, A., Badhan, A., Deyholos, M., & Kav, N. (2012). Proteomic Profiling of the Aleurone Layer of Mature Arabidopsis thaliana Seed. Plant Molecular Biology Reporter, 31(2), 464–469. https://doi.org/10.1007/s11105-012-0498-3

Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi

New Biotechnology / Nov 01, 2018

Badhan, A., Huang, J., Wang, Y., Abbott, D. W., Di Falco, M., Tsang, A., & McAllister, T. (2018). Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi. New Biotechnology, 46, 1–6. https://doi.org/10.1016/j.nbt.2018.05.003

Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transformed Mid-Infrared Spectroscopy (FT-IR)

Methods in Molecular Biology / Jan 01, 2017

Badhan, A., Wang, Y., & McAllister, T. A. (2017). Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transformed Mid-Infrared Spectroscopy (FT-IR). In Protein-Carbohydrate Interactions (pp. 209–214). Springer New York. https://doi.org/10.1007/978-1-4939-6899-2_16

Current Knowledge and Future Opportunities for Ruminant Enzymes

Enzymes in Farm Animal Nutrition / Mar 11, 2022

Rosser, C., Terry, S. A., Badhan, A., McAllister, T. A., & Beauchemin, K. A. (2022). Current Knowledge and Future Opportunities for Ruminant Enzymes. In Enzymes in Farm Animal Nutrition (pp. 153–169). CABI. https://doi.org/10.1079/9781789241563.0009

Blue Native Page in Combination with Mass Spectrometry as an Effective Tool to Study Multi-Enzyme Complex’s in Secretome of Aerobic Fungus

Annals of Chromatography and Separation Techniques / Jan 01, 2016

Badhan, A. (2016). Blue Native Page in Combination with Mass Spectrometry as an Effective Tool to Study Multi-Enzyme Complex’s in Secretome of Aerobic Fungus. Annals of Chromatography and Separation Techniques, 2(2), 1–2. https://doi.org/10.36876/acst.1019

From Plant Cell Walls to Biofuels—Arabidopsis thaliana Model

Handbook of Bioenergy Crop Plants / Mar 22, 2012

Pattathil, S., Avci, U., Biswal, A., & Badhan, A. (2012). From Plant Cell Walls to Biofuels—Arabidopsis thaliana Model. In Handbook of Bioenergy Crop Plants (pp. 75–97). CRC Press. https://doi.org/10.1201/b11711-7

Comparative analysis of functional diversity of rumen microbiome in bison and beef heifers

Applied and Environmental Microbiology / Dec 21, 2023

Nguyen, T. T. M., Badhan, A. K., Reid, I. D., Ribeiro, G., Gruninger, R., Tsang, A., Guan, L. L., & McAllister, T. (2023). Comparative analysis of functional diversity of rumen microbiome in bison and beef heifers. Applied and Environmental Microbiology, 89(12). https://doi.org/10.1128/aem.01320-23

Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transform Mid-Infrared Spectroscopy

Methods in Molecular Biology / Jan 01, 2023

Badhan, A., Wang, Y., & McAllister, T. A. (2023). Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transform Mid-Infrared Spectroscopy. In Carbohydrate-Protein Interactions (pp. 207–213). Springer US. https://doi.org/10.1007/978-1-0716-3151-5_15

New mechanistic insight into the digestion of complex dietary fibre by rumen microbiota using combinatorial high-resolution glycomic and transcriptomic analyses

Nov 13, 2020

Badhan, A., Low, K., Jones, D., Xing, X., Milani, M. R. M., OrtegoPolo, R., Abbott, D. W., Venketachalam, S., Hahn, M., & McAllister, T. (2020). New mechanistic insight into the digestion of complex dietary fibre by rumen microbiota using combinatorial high-resolution glycomic and transcriptomic analyses. https://doi.org/10.21203/rs.3.rs-104578/v1

PSX-15 Screening recombinant fibrolytic enzymes for improved ruminal fiber degradability of barley straw.

Journal of Animal Science / Dec 01, 2018

Ribeiro, G., Badhan, A., Huang, J., Beauchemin, K., Yang, W., Wang, Y., Tsang, A., & McAllister, T. (2018). PSX-15 Screening recombinant fibrolytic enzymes for improved ruminal fiber degradability of barley straw. Journal of Animal Science, 96(suppl_3), 413–414. https://doi.org/10.1093/jas/sky404.906

Education

Ph.D., Microbiology

Experience

Agriculture Agri-food Canada

Biologist / May, 2015Present

Links & Social Media

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