Michael Cukan
Bioconjugates, Analytical biochemistry method development
Research Interests
About
Publications
Optimization of humanized IgGs in glycoengineered Pichia pastoris
Nature Biotechnology / Jan 22, 2006
Li, H., Sethuraman, N., Stadheim, T. A., Zha, D., Prinz, B., Ballew, N., Bobrowicz, P., Choi, B.-K., Cook, W. J., Cukan, M., Houston-Cummings, N. R., Davidson, R., Gong, B., Hamilton, S. R., Hoopes, J. P., Jiang, Y., Kim, N., Mansfield, R., Nett, J. H., … Gerngross, T. U. (2006). Optimization of humanized IgGs in glycoengineered Pichia pastoris. Nature Biotechnology, 24(2), 210–215. https://doi.org/10.1038/nbt1178
Production of monoclonal antibodies by glycoengineered Pichia pastoris
Journal of Biotechnology / Feb 01, 2009
Potgieter, T. I., Cukan, M., Drummond, J. E., Houston-Cummings, N. R., Jiang, Y., Li, F., Lynaugh, H., Mallem, M., McKelvey, T. W., Mitchell, T., Nylen, A., Rittenhour, A., Stadheim, T. A., Zha, D., & d’Anjou, M. (2009). Production of monoclonal antibodies by glycoengineered Pichia pastoris. Journal of Biotechnology, 139(4), 318–325. https://doi.org/10.1016/j.jbiotec.2008.12.015
IgG2m4, an engineered antibody isotype with reduced Fc function
mAbs / Nov 01, 2009
An, Z., Forrest, G., Moore, R., Cukan, M., Haytko, P., Huang, L., Vitelli, S., Zhao, J. Z., Lu, P., Hua, J., Gibson, C. R., Harvey, B. R., Montgomery, D., Zaller, D., Wang, F., & Strohl, W. (2009). IgG2m4, an engineered antibody isotype with reduced Fc function. MAbs, 1(6), 572–579. https://doi.org/10.4161/mabs.1.6.10185
Glycoengineered Pichia produced anti-HER2 is comparable to trastuzumab in preclinical study
mAbs / May 01, 2011
Zhang, N., Liu, L., Dan Dumitru, C., Cummings, N. R. H., Cukan, M., Jiang, Y., Li, Y., Li, F., Mitchell, T., Mallem, M. R., Ou, Y., Patel, R. N., Vo, K., Wang, H., Burnina, I., Choi, B.-K., Huber, H. E., Stadheim, T. A., & Zha, D. (2011). Glycoengineered Pichia produced anti-HER2 is comparable to trastuzumab in preclinical study. MAbs, 3(3), 289–298. https://doi.org/10.4161/mabs.3.3.15532
Recombinant human lactoferrin expressed in glycoengineered Pichia pastoris: effect of terminal N-acetylneuraminic acid on in vitro secondary humoral immune response
Glycoconjugate Journal / Mar 26, 2008
Choi, B.-K., Actor, J. K., Rios, S., d’Anjou, M., Stadheim, T. A., Warburton, S., Giaccone, E., Cukan, M., Li, H., Kull, A., Sharkey, N., Gollnick, P., Kocięba, M., Artym, J., Zimecki, M., Kruzel, M. L., & Wildt, S. (2008). Recombinant human lactoferrin expressed in glycoengineered Pichia pastoris: effect of terminal N-acetylneuraminic acid on in vitro secondary humoral immune response. Glycoconjugate Journal, 25(6), 581–593. https://doi.org/10.1007/s10719-008-9123-y
Purification process development of a recombinant monoclonal antibody expressed in glycoengineered Pichia pastoris
Protein Expression and Purification / Mar 01, 2011
Jiang, Y., Li, F., Zha, D., Potgieter, T. I., Mitchell, T., Moore, R., Cukan, M., Houston-Cummings, N. R., Nylen, A., Drummond, J. E., McKelvey, T. W., d’Anjou, M., Stadheim, T. A., Sethuraman, N., & Li, H. (2011). Purification process development of a recombinant monoclonal antibody expressed in glycoengineered Pichia pastoris. Protein Expression and Purification, 76(1), 7–14. https://doi.org/10.1016/j.pep.2010.11.004
Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF
Glycomics / Feb 20, 2008
Gong, B., Cukan, M., Fisher, R., Li, H., Stadheim, T. A., & Gerngross, T. (2008). Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF. In Glycomics (pp. 213–223). Humana Press. https://doi.org/10.1007/978-1-59745-022-5_16
KPMW135, a Biosuperior CD3 Bispecific Version of Rituximab Created By a Novel Chemical Conjugation Technology Demonstrates Increased Anti-Tumor Activity By Adding T Cell-Mediated Cytotoxicity Activity to the Existing Mechanisms of Rituximab
Blood / Nov 05, 2020
Vidal, C., Cukan, M., Vaill, A., Bunin, A., Rossi, A. M., Iben, L., Trinh, D., McGrath, K., Berbasova, T., Romee, R., Alvarez, E., Rastelli, L., & Welsch, M. (2020). KPMW135, a Biosuperior CD3 Bispecific Version of Rituximab Created By a Novel Chemical Conjugation Technology Demonstrates Increased Anti-Tumor Activity By Adding T Cell-Mediated Cytotoxicity Activity to the Existing Mechanisms of Rituximab. Blood, 136(Supplement 1), 9–10. https://doi.org/10.1182/blood-2020-142113
635 A novel site-directed chemical conjugation technology confers antitumor activity via native Fc receptor to plasma immunoglobulin by attaching tumor binders
Regular and young investigator award abstracts / Nov 01, 2020
Vidal, C., Cukan, M., Varma, R., Iben, L., Berbasova, T., Vaill, A., Bunin, A., Rossi, A. M., Trinh, D., McGrath, K., Alvarez, E., Welsch, M., & Rastelli, L. (2020, November). 635 A novel site-directed chemical conjugation technology confers antitumor activity via native Fc receptor to plasma immunoglobulin by attaching tumor binders. Regular and Young Investigator Award Abstracts. https://doi.org/10.1136/jitc-2020-sitc2020.0635
Binding of DC-SIGN to glycoproteins expressed in glycoengineered Pichia pastoris
Journal of Immunological Methods / Dec 01, 2012
Cukan, M. C., Hopkins, D., Burnina, I., Button, M., Giaccone, E., Houston-Cummings, N. R., Jiang, Y., Li, F., Mallem, M., Mitchell, T., Moore, R., Nylen, A., Prinz, B., Rios, S., Sharkey, N., Zha, D., Hamilton, S., Li, H., & Stadheim, T. A. (2012). Binding of DC-SIGN to glycoproteins expressed in glycoengineered Pichia pastoris. Journal of Immunological Methods, 386(1–2), 34–42. https://doi.org/10.1016/j.jim.2012.08.015
A high-throughput purification of monoclonal antibodies from glycoengineered Pichia pastoris
Protein Expression and Purification / Nov 01, 2010
Jiang, Y., Li, F., Button, M., Cukan, M., Moore, R., Sharkey, N., & Li, H. (2010). A high-throughput purification of monoclonal antibodies from glycoengineered Pichia pastoris. Protein Expression and Purification, 74(1), 9–15. https://doi.org/10.1016/j.pep.2010.04.016
Education
Dartmouth College
PhD, Chemistry / January, 2019
Yale University
M.S., Cell Biology / August, 2002
Cornell University
A.B., Biochemistry / May, 1996
Experience
Olympus Biotech
2011 — August, 2013
Glycofi Inc
Research Biologist / August, 2004 — December, 2010
Agilix Corp
2002 — 2004
Cornell University
1996 — 1997
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