Michael Cukan

Bioconjugates, Analytical biochemistry method development

Research Interests

Immunology
Antibodies
Antibody drug conjugates
Applied Microbiology and Biotechnology
Biomedical Engineering
Molecular Medicine
Biotechnology
Bioengineering
Immunology and Allergy
Molecular Biology
Cell Biology
Biochemistry
Hematology

About

Michael Cukan is a highly experienced and educated scientist with a background in chemistry, cell biology, and biochemistry. He earned his PhD in Chemistry from Dartmouth College in 2019, his M.S. in Cell Biology from Yale University, and his A.B. in Biochemistry from Cornell University.

Publications

Optimization of humanized IgGs in glycoengineered Pichia pastoris

Nature Biotechnology / Jan 22, 2006

Li, H., Sethuraman, N., Stadheim, T. A., Zha, D., Prinz, B., Ballew, N., Bobrowicz, P., Choi, B.-K., Cook, W. J., Cukan, M., Houston-Cummings, N. R., Davidson, R., Gong, B., Hamilton, S. R., Hoopes, J. P., Jiang, Y., Kim, N., Mansfield, R., Nett, J. H., … Gerngross, T. U. (2006). Optimization of humanized IgGs in glycoengineered Pichia pastoris. Nature Biotechnology, 24(2), 210–215. https://doi.org/10.1038/nbt1178

Production of monoclonal antibodies by glycoengineered Pichia pastoris

Journal of Biotechnology / Feb 01, 2009

Potgieter, T. I., Cukan, M., Drummond, J. E., Houston-Cummings, N. R., Jiang, Y., Li, F., Lynaugh, H., Mallem, M., McKelvey, T. W., Mitchell, T., Nylen, A., Rittenhour, A., Stadheim, T. A., Zha, D., & d’Anjou, M. (2009). Production of monoclonal antibodies by glycoengineered Pichia pastoris. Journal of Biotechnology, 139(4), 318–325. https://doi.org/10.1016/j.jbiotec.2008.12.015

IgG2m4, an engineered antibody isotype with reduced Fc function

mAbs / Nov 01, 2009

An, Z., Forrest, G., Moore, R., Cukan, M., Haytko, P., Huang, L., Vitelli, S., Zhao, J. Z., Lu, P., Hua, J., Gibson, C. R., Harvey, B. R., Montgomery, D., Zaller, D., Wang, F., & Strohl, W. (2009). IgG2m4, an engineered antibody isotype with reduced Fc function. MAbs, 1(6), 572–579. https://doi.org/10.4161/mabs.1.6.10185

Glycoengineered Pichia produced anti-HER2 is comparable to trastuzumab in preclinical study

mAbs / May 01, 2011

Zhang, N., Liu, L., Dan Dumitru, C., Cummings, N. R. H., Cukan, M., Jiang, Y., Li, Y., Li, F., Mitchell, T., Mallem, M. R., Ou, Y., Patel, R. N., Vo, K., Wang, H., Burnina, I., Choi, B.-K., Huber, H. E., Stadheim, T. A., & Zha, D. (2011). Glycoengineered Pichia produced anti-HER2 is comparable to trastuzumab in preclinical study. MAbs, 3(3), 289–298. https://doi.org/10.4161/mabs.3.3.15532

Recombinant human lactoferrin expressed in glycoengineered Pichia pastoris: effect of terminal N-acetylneuraminic acid on in vitro secondary humoral immune response

Glycoconjugate Journal / Mar 26, 2008

Choi, B.-K., Actor, J. K., Rios, S., d’Anjou, M., Stadheim, T. A., Warburton, S., Giaccone, E., Cukan, M., Li, H., Kull, A., Sharkey, N., Gollnick, P., Kocięba, M., Artym, J., Zimecki, M., Kruzel, M. L., & Wildt, S. (2008). Recombinant human lactoferrin expressed in glycoengineered Pichia pastoris: effect of terminal N-acetylneuraminic acid on in vitro secondary humoral immune response. Glycoconjugate Journal, 25(6), 581–593. https://doi.org/10.1007/s10719-008-9123-y

Purification process development of a recombinant monoclonal antibody expressed in glycoengineered Pichia pastoris

Protein Expression and Purification / Mar 01, 2011

Jiang, Y., Li, F., Zha, D., Potgieter, T. I., Mitchell, T., Moore, R., Cukan, M., Houston-Cummings, N. R., Nylen, A., Drummond, J. E., McKelvey, T. W., d’Anjou, M., Stadheim, T. A., Sethuraman, N., & Li, H. (2011). Purification process development of a recombinant monoclonal antibody expressed in glycoengineered Pichia pastoris. Protein Expression and Purification, 76(1), 7–14. https://doi.org/10.1016/j.pep.2010.11.004

Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF

Glycomics / Feb 20, 2008

Gong, B., Cukan, M., Fisher, R., Li, H., Stadheim, T. A., & Gerngross, T. (2008). Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF. In Glycomics (pp. 213–223). Humana Press. https://doi.org/10.1007/978-1-59745-022-5_16

KPMW135, a Biosuperior CD3 Bispecific Version of Rituximab Created By a Novel Chemical Conjugation Technology Demonstrates Increased Anti-Tumor Activity By Adding T Cell-Mediated Cytotoxicity Activity to the Existing Mechanisms of Rituximab

Blood / Nov 05, 2020

Vidal, C., Cukan, M., Vaill, A., Bunin, A., Rossi, A. M., Iben, L., Trinh, D., McGrath, K., Berbasova, T., Romee, R., Alvarez, E., Rastelli, L., & Welsch, M. (2020). KPMW135, a Biosuperior CD3 Bispecific Version of Rituximab Created By a Novel Chemical Conjugation Technology Demonstrates Increased Anti-Tumor Activity By Adding T Cell-Mediated Cytotoxicity Activity to the Existing Mechanisms of Rituximab. Blood, 136(Supplement 1), 9–10. https://doi.org/10.1182/blood-2020-142113

635 A novel site-directed chemical conjugation technology confers antitumor activity via native Fc receptor to plasma immunoglobulin by attaching tumor binders

Regular and young investigator award abstracts / Nov 01, 2020

Vidal, C., Cukan, M., Varma, R., Iben, L., Berbasova, T., Vaill, A., Bunin, A., Rossi, A. M., Trinh, D., McGrath, K., Alvarez, E., Welsch, M., & Rastelli, L. (2020, November). 635 A novel site-directed chemical conjugation technology confers antitumor activity via native Fc receptor to plasma immunoglobulin by attaching tumor binders. Regular and Young Investigator Award Abstracts. https://doi.org/10.1136/jitc-2020-sitc2020.0635

Binding of DC-SIGN to glycoproteins expressed in glycoengineered Pichia pastoris

Journal of Immunological Methods / Dec 01, 2012

Cukan, M. C., Hopkins, D., Burnina, I., Button, M., Giaccone, E., Houston-Cummings, N. R., Jiang, Y., Li, F., Mallem, M., Mitchell, T., Moore, R., Nylen, A., Prinz, B., Rios, S., Sharkey, N., Zha, D., Hamilton, S., Li, H., & Stadheim, T. A. (2012). Binding of DC-SIGN to glycoproteins expressed in glycoengineered Pichia pastoris. Journal of Immunological Methods, 386(1–2), 34–42. https://doi.org/10.1016/j.jim.2012.08.015

A high-throughput purification of monoclonal antibodies from glycoengineered Pichia pastoris

Protein Expression and Purification / Nov 01, 2010

Jiang, Y., Li, F., Button, M., Cukan, M., Moore, R., Sharkey, N., & Li, H. (2010). A high-throughput purification of monoclonal antibodies from glycoengineered Pichia pastoris. Protein Expression and Purification, 74(1), 9–15. https://doi.org/10.1016/j.pep.2010.04.016

Education

Dartmouth College

PhD, Chemistry / January, 2019

Hanover, New Hampshire, United States of America

Yale University

M.S., Cell Biology / August, 2002

New Haven

Cornell University

A.B., Biochemistry / May, 1996

Ithaca

Experience

Olympus Biotech

2011August, 2013

Glycofi Inc

Research Biologist / August, 2004December, 2010

Agilix Corp

20022004

Cornell University

19961997

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